RESUMO
BACKGROUND: Resveratrol and Quercetin as powerful herbal antioxidant reduce kidney complications of diabetes. The purpose of this study was to evaluate the protective effects of Quercetin and Resveratrol on aging markers in high glucose condition. METHODS AND RESULTS: In this study, HEK-293 cells and kidney tissue of male Wistar rats were used in hyperglycemia condition. Quercetin and Resveratrol for cell culture and in vivo evaluation were used. Amount of methylglyoxal and the gene and protein expression of the Senescence Marker Protein30 (SMP30) were measured using qRT-PCR and ELISA, respectively. High glucose condition in kidney cell and tissue caused increase in glyoxal amount compared to control sample. Treatment with Resveratrol and/or Quercetin significantly decreased the amount of methylglyoxal in a dose-dependent manner. SMP30 expression significantly decreased compare to control group while treatment with Resveratrol and/or Quercetin considerably increased expression level of the protein. Six weeks treatment with Quercetin and Resveratrol had more beneficial effects on aging markers compared with three weeks. CONCLUSIONS: According to the results, it can be concluded that resveratrol and quercetin, as potent herbal antioxidants, may have significant effects in reducing the complications of diabetes in kidneys, as well as preventing the aging of the kidney cells.
Assuntos
Biomarcadores , Glicemia/efeitos dos fármacos , Senescência Celular/efeitos dos fármacos , Rim/efeitos dos fármacos , Rim/metabolismo , Quercetina/farmacologia , Resveratrol/farmacologia , Animais , Antioxidantes/farmacologia , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/metabolismo , Hidrolases de Éster Carboxílico/genética , Hidrolases de Éster Carboxílico/metabolismo , Nefropatias Diabéticas/tratamento farmacológico , Nefropatias Diabéticas/etiologia , Nefropatias Diabéticas/metabolismo , Nefropatias Diabéticas/patologia , Modelos Animais de Doenças , Glucose/metabolismo , Glucose/farmacologia , Células HEK293 , Humanos , Imuno-Histoquímica , Estresse Oxidativo/efeitos dos fármacos , Aldeído Pirúvico/metabolismo , RatosRESUMO
BACKGROUND: Hyperglycemia-mediated oxidative stress implicates in etiology of kidney cell aging and diabetic nephropathy. We evaluated the effects of different doses of resveratrol and quercetin and their combination therapy on aging marker in human kidney cell culture under hyperglycemia condition. METHODS: Human embryonic kidney cell (HEK-293) was cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 100 mM (18 mg/L) for 24 h. The cells were treated with resveratrol (2.5, 5, 10 µm), quercetin (3, 6, 12 µm), and combination of these (R 2.5 µm, Q 3 µm) and (R 5 µm, Q 6 µm) and (R 10 µm, Q 12 µm) for 48 h, and then, cells were lysed to access RNA and lysate. RESULTS: The analysis of data showed that beta-galactosidase enzyme gene expression as an aging marker in all treatment groups has reduced in a dose-dependent manner. Gene expression of Sirtuin1 and thioredoxin (Trx) in all treated groups in comparison to control group increased in a dose-dependent fashion. Trx interacting protein (TXNIP) gene expression decreased in a dose-dependent manner in all treated groups, especially in resveratrol and combination therapy. CONCLUSIONS: According to the results of this research, quercetin, resveratrol, and especially combination treatments with increased expression levels of antioxidants, can reduce aging markers in HEK cell line in hyperglycemia conditions. These results lead us to use flavonoids such as resveratrol for anti-aging potential.
